FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54051366
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA1347310.24926474568653825No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG1151850.21310284739149793No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG742340.1373397297674216No Hit
ATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA552720.10225828520226482No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG63850.018.14685226
ATATAGC83250.015.3200046
GGGAGGT354200.014.0272143
GGAGGTC357250.013.813434
GCTAAGT88300.013.75787714
TTGTCGT86950.013.63681327
TGTCGTT85850.013.49766228
GTCGTTC88400.013.10822129
TCGTTCC93550.012.50698930
TATAGCT103850.012.4766897
GAGGTCA413800.012.2359665
TACAATA111950.012.1137242
AGCTTAG316850.012.07676445
CAATATA122150.011.7480134
CGTTCCC97850.011.72851931
TAAGTGC108000.011.64522616
TGGGAGG338850.011.5286062
GTCACTC428700.011.4917068
GGTCACT425600.011.4694217
ATAGGGG332600.011.37980338
Produced by FastQC (version 0.11.5)