FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46389835
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGC514960.11100707730475867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG18900.018.331436
AGACCGC92350.017.493154
CAGACCG104150.015.770453
GACCGCG103800.015.6068465
GTCCGTT36650.015.1083231
ACCGCGT109700.014.8275396
TCGACGA24800.014.7867387
CCCGTAT35650.014.4587611
CTCGCGT12800.014.4197921
GCGGACT63450.014.2197912
CGTTACG12700.013.99485121
CGGTATA35150.013.95312220
GAACGAT115900.013.91891745
CCCCGCA253500.013.83099235
AGACGCT269050.013.80019741
CCTACTC72900.013.67112418
GTTCCTA73300.013.65787615
CCGCGTT119350.013.6286347
AAGCGGC260100.013.477735528
AAGCGCG14500.013.34315418
Produced by FastQC (version 0.11.5)