FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46475680
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG29650.018.2116376
CGGTATA39750.017.37655420
GTCCGTT41000.016.145491
CGTTACG17150.015.87398821
TCGACGA38250.014.528747
CCCGTAT36650.014.498591
CCGTACG64800.014.3059714
CGTACGC65050.014.1124815
GTTCGAC36250.014.0278445
CGAACGA29000.013.96632
ACGAACG29850.013.86991431
CTCCGAT53800.013.852671
TTCGGCG70100.013.83346744
AAGCGGC193200.013.71918128
CTCCGGT66800.013.4825271
AGACGCT198850.013.464807541
AGACCGC65850.013.4628064
CGACGAG38600.013.1729678
CTCCGTT71100.013.1421441
AACGCTA86800.013.14185723
Produced by FastQC (version 0.11.5)