FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42126383
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG428540.10172722400591573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT227900.013.2695563
AGCTTAG158050.012.88350145
ATAGGGG162850.012.37939738
GGCATAG166350.012.25419235
GGAGGTC248350.012.2221974
GAGCTTA183150.011.20384744
GGTCACT267000.011.1404977
TGGCATA184900.010.9882934
ACTCTCC280450.010.71843311
GTCACTC284050.010.6460618
CATAGGG193850.010.62024637
TGGGAGG219300.09.9525542
GCATAGG209150.09.89712936
GTTGTCG13150.09.75277926
TCACTCT325400.09.5697579
TCTCCCC313700.09.5464713
GCTCTGT316650.09.46463922
TAGGGGA309550.09.45639839
TGCCCGT114700.09.23925144
TCAGACG73900.09.1952582
Produced by FastQC (version 0.11.5)