FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50439350
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT960720.19047033714748504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG218800.021.6197935
AGACCGC221050.021.4403934
ACCGCGT221800.021.36696
CCGCGTT226600.020.9440697
CGCGTTC227300.020.8597778
CAGACCG230000.020.733223
GAACGAT229300.020.64133845
TTCGGCG64350.018.78216744
TCGGCGC68450.017.88775645
TCAGACC272050.017.8513932
AGAACGA273350.017.36450844
GCGTTCT274750.017.355489
GTTCCTA102600.017.3407415
CCTACTC107300.016.74888618
CCGTACG74000.016.7486044
CTCAGAC326150.016.3306241
CGTACGC76600.016.2388765
AATACGG292150.016.18430932
TACGGAG294400.016.03054834
ATACGGA295750.015.98771733
Produced by FastQC (version 0.11.5)