FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50439350
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG138800.012.73537838
GGCATAG139350.012.35112835
AGCTTAG145550.011.95015945
GGGAGGT221900.011.4148883
GTTCTAT160650.011.3041371
CATAGGG160100.010.9229137
GGAGGTC233950.010.7116634
GCATAGG164350.010.63936836
TGGCATA169850.010.27028534
GAGCTTA170500.010.217444
GGTCACT237350.010.1467427
ACTCTCC246350.010.0263911
GTCACTC246600.010.023298
GCGTTCG23550.09.73945715
GTTGTCG21850.09.27663426
TCTATAG272200.09.2229563
CACTCTC264600.09.12158710
CCGCGTT26900.09.02801313
CGTAGGG67850.08.94603137
GCGTAGG67700.08.93161436
Produced by FastQC (version 0.11.5)