FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45547540
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG102450.012.14484845
ATAGGGG105500.011.98589438
GGCATAG109550.011.33739535
CATAGGG118200.010.71710537
GGGAGGT193600.010.5177343
ACCCGGC60850.010.5011465
GGTCACT176100.010.2597117
GTCACTC180950.010.1961038
TCAGACG74150.010.1347792
GAGCTTA127050.09.95269244
GCATAGG129900.09.66521736
ACTCTCC193550.09.60209111
TGGCATA131500.09.56481234
CGTGGCG108250.09.1662061
TAGGCTA70800.08.898231510
ATAGCTC63300.08.88627939
GGAGGTC224050.08.7167324
GTGGCGA108700.08.714282
TATCCTA77550.08.6749775
CAGACGT82150.08.6548293
Produced by FastQC (version 0.11.5)