FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44148159
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA840940.19048132901759277No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG758160.17173083027086133No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG643440.1457456017588412No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCTA137550.017.78861445
GTTGTCG40200.015.72406226
GGGAGGT367900.014.9029373
CATGCAT96200.014.6376019
GGAGGTC375000.014.4751644
GAGGTCA383950.014.3433665
GGTCACT384400.013.8640347
GTCACTC389600.013.6026268
AGCTTAG277850.013.41213745
AGGTCAC418150.013.1393056
ATACATG108000.013.129666
ACTCTCC410950.012.94710211
GGCATAG287550.012.84994235
ATAGGGG291400.012.8421538
ATATAGC63600.012.740376
TGTCGTT50800.012.57554328
GCTAAGT53300.012.32033914
TCACTCT436750.012.2836139
TGGCATA304500.012.26671134
TGGGAGG326300.012.1537332
Produced by FastQC (version 0.11.5)