FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49203995
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2751900.5592838548983675No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA694060.14105765192440167No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG548170.11140762045846074No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT472950.028.77748945
AGACCGC554950.024.7395784
CAGACCG560350.024.5613983
ACCGCGT557700.024.5530416
GACCGCG562050.024.3790255
CCGCGTT569200.024.1399867
AGAACGA566100.024.07372344
CGCGTTC573300.023.9791188
TCAGACC604350.023.0375212
GCGTTCT613500.022.5142279
CTCAGAC655700.022.058491
AAGAACG631400.021.58731843
AATACGG634350.021.401832
ATACGGA638400.021.23783333
TACGGAG638400.021.11800634
CAATACG653800.020.87179831
CGTTCTC681600.020.46284710
ACGGAGA664800.020.29969235
CGGAGAG675600.019.87527536
CCCAATA693750.019.79961229
Produced by FastQC (version 0.11.5)