FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47197398
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2252450.4772402919330425No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC599640.12704937674742153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT356850.029.45167445
AGACCGC401150.026.3949474
GACCGCG401850.026.2929765
ACCGCGT404600.026.108716
CAGACCG406100.026.0842953
AGAACGA408100.025.79682444
CCGCGTT413600.025.6058587
CGCGTTC419250.025.2876138
TCAGACC444850.024.054922
AATACGG447650.023.50739132
ATACGGA454600.023.1183133
TACGGAG455550.023.05528334
GCGTTCT470950.022.6119169
CAATACG478700.022.0484331
AAGAACG481400.021.89672543
CTCAGAC520350.021.8463841
CGGAGAG483150.021.73824736
ACGGAGA489800.021.42932535
CCCAATA505050.020.93373529
TTCGGCG141950.020.0826544
Produced by FastQC (version 0.11.5)