FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53593586
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG158350.015.1188445
GGCATAG176150.013.53991935
ATAGGGG187000.012.99493838
GAGCTTA195150.012.40619644
GGGAGGT296650.012.3150133
CATAGGG202950.012.02908937
TGGCATA200650.011.99909334
GGAGGTC311900.011.4604514
GCATAGG216450.011.26844936
GTTGTCG23450.010.74616426
TAGGGGA336850.010.08659639
ACTCTCC362650.09.97516111
TAGGCTA81350.09.899816510
GGTCACT354600.09.8012657
GTCACTC367600.09.75431258
GCGTAGG101700.09.73473636
CGTAGGG102450.09.641537
GGCGTAG104200.09.5227835
GCTCTGT376500.09.32863822
TATCCTA92800.09.308435
Produced by FastQC (version 0.11.5)