FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52391508
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG163100.012.92778545
ATAGGGG164500.012.856235538
GGCATAG169450.012.58688335
GGGAGGT266100.012.1359363
GAGCTTA191600.011.02767944
TGGCATA195300.010.95543734
GTCACTC285000.010.9275578
GGTCACT286300.010.7758137
CATAGGG199250.010.7156737
GGAGGTC302450.010.6922994
ACTCTCC309350.010.12513111
GCATAGG215050.010.04345736
GTTGTCG22700.09.71296126
TCAGACG72550.08.9332892
GCGTAGG102450.08.91590736
CGTAGGG101950.08.87136137
TAGGGGA340650.08.86335339
GGCGTAG103150.08.85540135
CACTCTC357850.08.70895610
GCTCTGT360750.08.65025722
Produced by FastQC (version 0.11.5)