FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45622810
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT784490.1719512673594634No Hit
CTGGGCTTGTGATTCACGTTGCAGATGTAGGTCTGGGTGCCCAAGCTGCTG494570.10840410750674936No Hit
CTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTC479040.1050001084983586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC184300.022.8027824
ACCGCGT188700.022.2472746
CAGACCG195850.021.527093
CCGCGTT198550.021.1549077
GAACGAT192650.021.00984245
CGCGTTC201400.020.9111718
GACCGCG202100.020.683285
TCAGACC226500.019.132872
GCGTTCT232050.018.2169049
AGAACGA224050.018.09544644
CTCAGAC278250.017.1001971
AATACGG235700.017.05016932
TTCACGC163200.016.763577
ATACGGA240150.016.7525733
CGTTACG18250.016.61769521
TACGGAG240600.016.59045634
AACGCTA164350.016.08823823
TCACGCT170850.016.0522988
CAATACG255300.015.987555531
ACGCTAC156600.015.722445524
Produced by FastQC (version 0.11.5)