FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45622810
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCT565620.12397745776728791No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAGGA267450.012.9296751
ATAGGGG114600.012.44551738
AGCGCTC68650.012.3204639
GGCATAG114250.012.08921435
GCGCTCT70050.011.68888110
AGCTTAG120300.011.42535245
GGGAGGT180100.011.3682993
CGTCAGT116950.010.8926591
GGAGGTC183350.010.835434
GCAGCGC79350.010.6592437
TGGCATA132650.010.63274234
CGCTCTC77000.010.57570811
CAGCGCT79400.010.5108628
GAGCTTA133800.010.22205444
GTTGTCG17950.09.90127126
CATAGGG141150.09.89738537
GCATAGG142900.09.77617736
ATAACGC89350.09.61779839
CGTAACG10700.09.25064442
TAACGCC94100.09.15614540
Produced by FastQC (version 0.11.5)