FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51411567
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG47800.031.1165076
GTTCGAC53250.027.974075
TCGACGA54450.027.6854747
CGACGAG57600.025.7808068
CGGTATA77100.025.35995720
AGTTCGA69850.021.6803324
ATGACGG87150.021.17017716
GACGGTA96850.020.07230218
AGACCGC86500.019.8223974
GACGAGG95100.018.6195349
CGAGGAT92650.018.48050511
ACGGTAT108850.018.14885119
CAGACCG95750.018.048443
GACCGCG95550.017.6858735
ATAGAGT119450.017.34808324
GTATAGA120100.017.1981922
AACGACG27300.017.0602616
ACGAGGA108550.016.83064710
CGCGTGA8500.016.6796381
TGACGGT112750.016.58297517
Produced by FastQC (version 0.11.5)