FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51411567
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG32150.013.78704226
AACTCTA122250.013.32625545
GGGAGGT207350.012.8549143
GACGCAA66750.011.73163844
GGAGGTC231150.011.6877194
ATAGGGG181450.011.41042238
GGCATAG179850.011.19932635
AGCTTAG187750.010.8104845
ACTCTCC239650.010.79571311
GGTCACT237750.010.739367
ATATAGC55450.010.4260416
TAATCGG55800.010.1185944
GAGCTTA200550.010.05347144
ACGCAAG80200.010.04447145
TATATAC71950.010.0363847
TGTCGTT45750.09.93471528
CGTGGCG63400.09.8260041
CATGCAT93150.09.8052939
GTCACTC268200.09.6881358
TGGCATA210000.09.6879234
Produced by FastQC (version 0.11.5)