FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50202889
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT714600.14234240583246116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC211900.020.1608074
CAGACCG220500.019.5275273
GACCGCG249600.017.0976435
GTCCGTT41200.016.3886781
GAACGAT215850.016.3331645
ACCGCGT262200.016.3103376
TTCGACG14850.015.60357956
CCGCGTT274350.015.5880557
CGCGTTC281150.015.2430588
GCGTTCT282550.015.1834559
CGTTACG14750.014.94887521
CTCAGAC313600.014.8851291
CCGTACG59450.014.8336534
ACGTTCG12900.014.8232266
TCAGACC301550.014.7191152
CGTACGC61700.014.4020865
CCGGCTT208150.014.3527662
CCCGTAT30300.014.18765451
TTCACGC186300.014.1644587
GCCGGCT196550.014.0504871
Produced by FastQC (version 0.11.5)