FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45239933
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT665490.14710233987304977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC151450.021.383054
ACCGCGT153700.020.947756
CAGACCG156350.020.9144693
GACCGCG160850.020.1335495
CCGCGTT161400.019.9483837
GAACGAT162050.019.90942645
CGCGTTC163150.019.7615138
TTCGACG18750.017.6395576
TCGGCGC85600.017.45231245
TTCGGCG87200.017.10628544
TCAGACC198900.016.8815822
CGTTACG9900.016.13606521
AGAACGA203050.016.02226644
AATACGG202800.016.00901832
CCGTACG94250.015.97433954
GTTCCTA75500.015.91310315
CGTACGC95300.015.7984255
ATACGGA206400.015.79517833
TACGGAG207550.015.599256534
GCGTTCT212000.015.2928759
Produced by FastQC (version 0.11.5)