FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45239933
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG496280.10969954354264849No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG184850.013.76655945
GGCATAG196700.012.94497335
GGTCACT276850.012.7398517
GGGAGGT305650.012.7236393
GGAGGTC298400.012.2478514
GTCACTC292050.012.2350298
ATAGGGG213450.012.18358638
GAGGTCA307300.011.9883355
TGGCATA215700.011.86738634
GAGCTTA217450.011.81651644
ACCCGGC78750.011.4309445
ACTCTCC315250.011.3501111
TCACTCT334800.010.92836959
CACTCTC333200.010.7384610
GTTGTCG21350.010.64319626
CATAGGG242200.010.57961337
GCATAGG252300.010.14652436
ATAGCTC83650.010.1392839
TAGCGTA7750.09.86955137
TCAGACG93750.09.86667252
Produced by FastQC (version 0.11.5)