FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200838

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200838
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50730496
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT834450.16448686013241426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT167100.024.45665445
AGACCGC190450.021.701514
CAGACCG202750.020.5403443
AGAACGA207750.019.7687644
GACCGCG209700.019.7093375
ACCGCGT217900.018.9986156
CCGCGTT221700.018.6625847
ATACGGA219750.018.64494933
TACGGAG219300.018.63086334
CGCGTTC223000.018.5739678
AATACGG219900.018.56060832
AAGAACG230450.017.82141143
CAATACG239950.017.17848631
GCGTTCT248300.016.7267179
GTCCGTT41650.016.5337311
TCAGACC257900.016.4806882
TTCGACG15200.016.4301196
ACGGAGA253400.016.23919935
CTCAGAC294250.015.8543091
CCCAATA266550.015.47263829
Produced by FastQC (version 0.11.5)