FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43535205
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC85650.018.8863684
GACCGCG89550.018.1894665
ACCGCGT89750.018.0486626
TTCGGCG60400.017.3609444
CAGACCG96100.017.2071293
CCGCGTT100050.016.2130667
CGCGTTC103300.015.6812318
GTCCGTT36200.015.5477231
GAACGAT105950.015.24859445
CCGTACG70000.015.0094854
CGTACGC70400.014.7963735
TCGGCGC71350.014.69596945
CTCGGTT74650.014.6568891
CTCCGTT61750.014.1823431
CTCCGAT53400.013.7861141
CTACACG41550.013.75526645
TTCGCGC13100.013.5675917
CTCCGGT62100.013.5223641
TACGCCA77200.013.5222097
CCCGTAT33300.013.3861691
Produced by FastQC (version 0.11.5)