FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48977524
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG511890.10451528746124447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG28700.016.22610926
GGGAGGT228150.014.7185713
GGCATAG194750.013.58476735
AGCTTAG199150.013.44108745
GGAGGTC249000.013.4302584
ATAGGGG203900.013.41784238
GTCGTTC38200.012.54195729
ATATAGC42950.012.5229136
GAGCTTA213950.012.34528744
TGTCGTT38900.012.31665828
TGGCATA221700.012.06389234
TGGGAGG247550.011.4200732
GCTAAGT42500.010.90745914
ACTCTCC298950.010.86243711
TCGTTCC43450.010.81936130
CATAGGG248250.010.78459937
CTGGGAG301800.010.651771
TCAGACG80050.010.6285162
ATAATCG57650.010.5800393
GTAACGA50900.010.565818
Produced by FastQC (version 0.11.5)