FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44426119
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1015720.22863126981674903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT250200.019.4598345
AGACCGC261150.018.7214164
CAGACCG270050.018.2293913
GACCGCG279950.017.4481095
CGTACGC58750.017.4249925
CCGTACG58600.017.3160154
ACCGCGT283400.017.267466
CCGCGTT296150.016.5468447
CGCGTTC296250.016.5412588
GTCCGTT38150.016.527351
CGAGCTA40650.016.43860211
AGAACGA299800.016.32287644
TCAGACC316150.015.9626722
GCGTTCT309700.015.8592099
TACGAGC43450.015.8453089
GTTCCTA75200.015.617866515
CCCGTAT34250.015.4506581
CTCAGAC354350.015.3406761
TACGCCA68950.015.1083067
TTCGGCG66400.015.04471444
Produced by FastQC (version 0.11.5)