FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51773553
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGC752420.14532902541959986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACG21650.018.08352521
GACCGCG98650.017.3113695
AGACCGC99600.017.2592034
ACCGCGT103250.016.5615336
TTCGACG21550.016.3919056
AGACGCT355350.016.27708641
CTCGGTT116050.016.111231
CCCCGCA350950.015.98363235
CCGCGTT108200.015.8870327
CAGACCG111350.015.7594853
CCGTACG68000.015.7501524
CGCGTTC109150.015.6868858
AAGCGGC354750.015.57924328
CCGCAGA384700.015.35926937
GCAGACG375500.015.35098239
CGTACGC70450.015.2662935
CCCCCGC380750.015.21142934
CAGACGC394950.014.71462740
GAACGAT116100.014.67212945
GCCCCCG395850.014.63141733
Produced by FastQC (version 0.11.5)