FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51773553
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG153500.011.87481345
ATAGGGG158700.011.7680138
GGCATAG157750.011.43883235
GGGAGGT235800.010.7423873
CATAGGG180250.010.02397737
GGTCACT238650.09.9636597
GAGCTTA185250.09.9242144
TGGCATA184000.09.88027434
GCATAGG187250.09.69689336
GGAGGTC256050.09.6541324
GTCACTC254750.09.5463068
ACTCTCC263300.09.31384211
TAATCGG41700.09.169664
GAGGTCA301950.08.5446345
CACTCTC293100.08.31299310
TCACTCT307450.08.2759819
GCGTTCG23050.08.199820515
GACGCAA47900.08.12723244
CGTAACG16950.08.09822242
CCGCGTT22650.08.046576513
Produced by FastQC (version 0.11.5)