FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50252253
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG649320.12921211711642064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT357150.013.4925413
AGCTTAG235450.013.31888645
GGCATAG245100.012.7423735
ATAGGGG251050.012.69139138
GTTGTCG27850.012.59978126
GGTCACT355400.012.3827237
GTCACTC360400.012.2883648
GGAGGTC376300.012.1841414
GAGCTTA264850.011.87699244
ACTCTCC385500.011.5522611
TGGCATA273800.011.52175434
GAGGTCA411200.011.3634015
AGGTCAC409500.011.2897126
TGGGAGG318250.010.6740382
TCACTCT424600.010.6050599
CACTCTC417900.010.59198910
CATAGGG317550.010.04069837
CTGGGAG382750.09.7356361
GCATAGG330500.09.61318536
GCTCTGT473150.09.55869622
Produced by FastQC (version 0.11.5)