FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50410964
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG129550.011.11480738
AGCTTAG137150.010.48441345
GGCATAG138550.010.37645435
GGGAGGT200050.010.1579893
GAGCTTA152650.09.58163744
GGTCACT205750.09.4597487
GTCACTC208900.09.2955648
CATAGGG157050.09.096909537
TAATCGG46550.09.0402494
GGAGGTC227150.08.8371174
GCATAGG163550.08.72157136
TGGCATA165950.08.69031334
GTTGTCG22450.08.51850326
ATCGGTT53100.08.4749836
ACTCTCC237250.08.41223211
TCAGACG73500.08.2361272
AGGTCAC258150.08.1497236
GAGGTCA258700.08.0201135
ATAATCG55150.07.99777273
CGTGGCG89100.07.95599171
Produced by FastQC (version 0.11.5)