FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49059732
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1459030.2973986894180343No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1250440.25488113143382035No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG533140.10867160872383079No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT317550.023.049581
CCGGCTT391150.021.3150022
TTCGACG33900.020.5712016
TTCACGC413650.020.2023647
TCACGCT421850.019.7084738
CGGCTTC454900.018.8570693
AGACCGC338300.018.2808364
ACGCTCA464150.018.03839710
CAGACCG346500.017.887173
GACCGCG351000.017.5419255
ACCGCGT354200.017.452836
CGTTACG20250.017.44683321
AACGCTA471350.017.38039423
ACGCTAC472700.017.32850524
ACGCGTC17650.017.07794440
CACGCTC493950.017.0366339
AAAACGC489750.016.7664621
CGCTACC488750.016.74570825
CCGCGTT369400.016.7277767
TCGACGA42200.016.684317
Produced by FastQC (version 0.11.5)