FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49059732
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA1101990.22462209944400022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG32350.015.0190526
GTTCTAT428300.013.633041
GGGAGGT257750.012.7060563
CACCGAA560050.012.23006845
GGAGGTC259600.012.1632624
GCACCGA567150.012.13255244
ATAGGGG193000.011.9629538
GGCATAG190000.011.79667835
AGCTTAG191850.011.77934745
TTCTATA614850.011.670582
TCTATAG607300.011.6267313
GGTCACT268650.011.4016337
AGGGATC189250.011.2036182
CTATAGT647900.010.92470554
TGTCGTT46300.010.88225628
ATATAGC55700.010.7899916
ATAGTGT672000.010.6551486
TATAGTG668150.010.6546725
GAGCTTA211300.010.64890344
GTCACTC291550.010.6209828
Produced by FastQC (version 0.11.5)