FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46989840
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG483270.10284563641842577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG172700.014.3868745
GGGAGGT269350.013.5697643
GGCATAG184500.013.44232635
ATAGGGG184850.013.344638
GTCACTC282950.012.4649828
GAGCTTA205800.012.083837544
TGGCATA210850.011.68785334
GGAGGTC316650.011.5711414
GTTGTCG24100.011.27898526
ACTCTCC313200.011.20368711
TCACTCT325850.011.2030789
GAGGTCA335150.010.9793445
GGTCACT322950.010.9697857
CATAGGG230250.010.7429237
GCATAGG243000.010.26167936
CACTCTC358000.09.80793710
AGGGATC128150.09.72917652
TGGGAGG279600.09.7138382
GCTCTGT364350.09.60618322
TCCAAGT377350.09.47169128
Produced by FastQC (version 0.11.5)