FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56987717
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG157050.013.48235545
ATAGGGG157800.013.47571638
GGCATAG159750.013.04386835
GGGAGGT270500.012.0665373
GAGCTTA178850.011.86423944
ACCCGGC79650.011.8058035
CATAGGG189100.011.06696237
GGTCACT275850.011.0173787
GTCACTC286800.010.8328848
TGGCATA194650.010.77451534
ACTCTCC288100.010.7220711
GGAGGTC297350.010.6747674
TCACCCG99850.010.4532763
GCATAGG201350.010.43835136
ATAGCTC90600.010.23242339
TCAGACG80600.09.9356332
CACTCTC317200.09.85854210
GTTGTCG16900.09.85314326
TAGCTCT99300.09.47177940
TCACTCT338600.09.4482169
Produced by FastQC (version 0.11.5)