FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43496460
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT3115450.7162536905302178No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGA1745920.4013935846733274No Hit
CCCAGCTTTATTTCCAACGTTGTGGAAAGGGACTTGTACATCATGGGGCAG1206960.2774846504750042No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1149250.26421690408828674No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG951610.21877872360187473No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA817740.18800150632948062No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG665690.15304463857518522No Hit
CCTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAGAGAAACCCACACATA650740.14960757726031038No Hit
CCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGAG616490.14173337324462726No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT596310.13709391522896344No Hit
GTAGGAATATGCAGGCGCAGACACGAGGCCCAGCTTCATCTTCAACGTTGT589430.1355121773128204No Hit
GTAGGAATATGCAGGCGCAGACACGAGGCCCAGCTTTATTTCCAACGTTGT530710.12201222812155288No Hit
GGCGGACTTTGGAGTGGGAAGCGGGGAATGTCTACGTGCGTATGCACGTGG516330.11870621195380039No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG496510.11414951929421384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG57300.032.1203466
GTTCGAC59150.030.5066245
TCGACGA59750.030.2384267
CGGTATA96850.025.74027420
CGACGAG73650.024.7147858
AGTTCGA75650.024.4773524
CAGACCG687950.023.6523933
AGACCGC687900.023.5952424
GACCGCG682400.023.587645
ACCGCGT683400.023.5864286
TCAGACC716300.023.0680712
CCGCGTT712100.022.6389737
CGCGTTC715000.022.4778988
AACGACG39750.021.73537616
GCGTTCT742600.021.7212439
CTCAGAC824500.021.4258631
CAGTTCG91550.020.8160633
CGTTCTC802400.020.43051510
CGAGGAT104150.020.19916311
TATAGAG135350.020.18057323
Produced by FastQC (version 0.11.5)