FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53015374
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1359780.25648786331300805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT280400.023.22665645
AGACCGC410300.021.83564
CAGACCG419500.021.4695383
GACCGCG423150.021.049925
ACCGCGT437550.020.4246
CCGCGTT449700.019.89727
CGCGTTC457650.019.576148
AGAACGA340600.019.18747144
TCAGACC485700.019.0020982
GCGTTCT489650.018.3657079
CTCAGAC539400.018.1005781
AAGAACG514750.017.40009743
TACGGAG526250.016.9387634
AATACGG527750.016.91619532
ACTCCCC535850.016.84955625
ATACGGA533550.016.75760733
CAATACG540750.016.5844131
CCTCTCA555350.016.53814519
CGTTCTC563900.016.33047910
ACGGAGA558950.015.9479535
Produced by FastQC (version 0.11.5)