FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53015374
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG679650.12819866176931996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG229900.014.62752545
GGGAGGT330150.014.2810193
ATAGGGG252900.013.34559138
GTTGTCG27400.013.29983426
GGCATAG259700.012.95946135
GTCACTC354950.012.9341148
GGAGGTC375050.012.7572194
GAGCTTA270500.012.51704644
GGTCACT369850.012.5046237
AGGTCAC394800.012.0505736
TGGCATA284350.011.961308534
ACTCTCC392050.011.77311511
GAGGTCA426300.011.4768755
TCACTCT411700.011.3753339
TGGGAGG334300.010.880472
CATAGGG320950.010.61349637
TATCCTA108550.010.4491325
CACTCTC442700.010.42103110
ATATAGC44400.010.3401566
GCATAGG332100.010.29023336
Produced by FastQC (version 0.11.5)