FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51983818
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT935790.18001563486544986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT196150.023.23880445
TTCGACG24150.022.452416
AGACCGC236300.020.4250034
CAGACCG246250.019.7824943
AGAACGA259550.018.5504944
CGGTATA40500.017.8910320
GACCGCG276800.017.3796335
ATACGGA278700.017.14668533
TACGGAG280900.016.99637234
AATACGG282300.016.95990232
AAGAACG285700.016.85257143
CCGCGTT287750.016.8107137
ACCGCGT289950.016.6831636
CGCGTTC294100.016.4477488
CAATACG295450.016.31167231
TCAGACC305600.016.1320082
CTCAGAC333900.015.9108721
ACGGAGA313150.015.2028835
GCGTTCT319400.015.1942159
CCCAATA330600.014.71407729
Produced by FastQC (version 0.11.5)