FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46015089
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA657130.14280750386030983No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG612710.13315414863154998No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG533760.11599673315855154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT296650.013.5401573
GGAGGTC297100.013.1156024
GGTCACT317250.011.98864657
AGGGATC163850.011.4900772
GTTGTCG21800.011.451550526
ATAGGGG236750.011.16018138
AGCTTAG237750.011.02678245
GGCATAG238900.011.00246635
GTCACTC361750.010.726198
TGGCATA249950.010.56111234
TGGGAGG265900.010.5442122
ACTCTCC384650.010.14017211
TCACTCT391550.010.1114479
CACTCTC390150.09.957190510
GAGCTTA264650.09.91483144
GCGTTCG22300.09.88932415
TCTCCCC385750.09.86000713
GCTCTGT387650.09.80526622
ACCCGGC60550.09.7377695
CCGCGTT23150.09.72177213
Produced by FastQC (version 0.11.5)