FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50805627
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG585910.1153238400148078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG204200.014.27500145
GGGAGGT315500.013.5827863
ATAGGGG227100.012.83288938
GGTCACT332800.012.5526057
GGCATAG233450.012.43377335
GTCACTC344300.012.1587988
GAGCTTA241150.012.143743544
GGAGGTC361450.012.1048534
GTTGTCG23250.011.99998226
TGGCATA258600.011.38108734
GAGGTCA386050.011.3567555
ACTCTCC370400.011.32062311
TCACTCT390000.010.8376659
CACTCTC396350.010.53362510
TGGGAGG298100.010.40628052
CACCCGG114550.010.0569561
CATAGGG293100.010.027143537
GCATAGG303200.09.67017436
ACCCGGG117050.09.4171162
GCTCTGT451800.09.41599822
Produced by FastQC (version 0.11.5)