FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200880

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200880
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46246843
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT733900.15869191330530388No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT155200.024.5595576
GACCGCG156350.024.3501825
AGACCGC162600.023.5799774
CCGCGTT164500.023.1164677
CGCGTTC165800.022.9487618
CAGACCG176400.021.8246773
GTTCCTA104250.019.52892715
GCGTTCT205250.018.6145089
TCAGACC214450.018.4362932
CTCAGAC251750.017.6668551
GAACGAT216950.016.97909745
GCACTAC78550.016.6735825
CCTACTC123550.016.6256918
ACGCGTC13400.016.5943740
CCGTACG42950.016.107124
CGTACGC43650.016.0546475
AAGCGCG14300.015.87222618
TCGGCGC42050.015.86429745
CGTTCTC260000.015.24760910
CATGCCC137150.014.9685857
Produced by FastQC (version 0.11.5)