FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45899647
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC78850.020.033064
TCACGCT182950.019.2461198
AACGCTA181850.018.632823
ACGCTAC182900.018.52583124
TTCACGC191500.018.3985757
CGCTACC188150.017.96108625
ACGCTCA199600.017.94501110
GACCGCG88250.017.7206465
CGTTACG23500.017.32914521
AAAACGC200650.017.0215521
CAGACCG95200.016.8997763
GAAAACG195500.016.88289620
ACCGCGT95750.016.4717776
AAACGCT214450.015.96816622
GTTCCTA81300.015.69111415
CACGCTC229300.015.4735129
CCGTACG48900.015.2771064
CCCGTAT31800.015.2318951
CCGCGTT104250.015.1503367
GCCGGCT127300.015.1491381
Produced by FastQC (version 0.11.5)