FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46624510
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT867960.18615959717324645No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG633670.1359092031208478No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC216650.021.8997334
CAGACCG225700.021.1212843
GACCGCG224150.020.976285
ACCGCGT229500.020.4970936
CCGCGTT243750.019.2809417
CGCGTTC247250.019.0535438
TCAGACC276950.017.7082672
TTCGACG18550.017.5851066
CTCAGAC310400.017.1714081
GAACGAT265650.016.85375845
GCGTTCT286950.016.4801779
CCGGCTT269150.016.3157232
GTCCGTT39300.016.0365681
GCCGGCT242550.015.8686791
TTCACGC268500.015.6267617
CGTTACG19800.015.34235421
TCACGCT278450.015.0279988
AGAACGA313800.014.33938644
AATACGG314550.014.10686132
TACGGAG312650.014.07630434
Produced by FastQC (version 0.11.5)