FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46624510
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA498070.10682578755251261No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG468940.10057800071250078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG32050.014.39150926
GGGAGGT195000.011.5961993
ATATAGC48700.011.5040916
ATAGGGG149400.011.05420138
GGAGGTC208700.010.5978014
TAATCGG47150.010.212144
GGCATAG158850.010.18408935
GTAACGA42350.010.09440418
AGCTTAG163100.010.02948545
AATCGGT51650.010.019385
GGTCACT215150.09.8721687
GCTAAGT46900.09.83470914
ATCGGTT52000.09.8221016
ATAATCG48100.09.7765463
GTCACTC222050.09.6464628
TGTCGTT48100.09.58935328
AGGGATC149400.09.3524272
GTCGTTC50650.09.28426129
GAGCTTA187000.09.03642344
TGGCATA187000.08.86760634
Produced by FastQC (version 0.11.5)