FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48130195
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1636980.34011497356285386No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG700010.1454409233122783No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA658830.13688496379455767No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG513040.10659420764864967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT345400.022.54874645
TTCGACG21100.018.2332386
AGACCGC432300.018.2151584
CAGACCG439500.017.9628243
AGAACGA436300.017.91274844
GACCGCG451750.017.3562035
CGTTACG26000.017.23157921
ACCGCGT460600.017.0617986
CCGCGTT470900.016.698167
CGCGTTC471450.016.6786798
TCAGACC495300.016.1874162
GCGTTCT488700.016.1498099
CTCAGAC527200.015.993661
AAGAACG509250.015.39092643
CCGTACG71700.014.7792324
AATACGG535950.014.61611832
TACGGAG537250.014.52619434
CGTACGC73850.014.5012885
ATACGGA540750.014.47385233
CGTTCTC555400.014.4276710
Produced by FastQC (version 0.11.5)