FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48130195
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA531040.1103340636787364No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG517260.10747099611792556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG177700.011.72692938
GGGAGGT247700.011.5349153
GTTGTCG27050.011.4770326
AGCTTAG180700.011.44748945
GGCATAG183050.011.1376635
GGAGGTC265650.010.86603454
GGTCACT256600.010.83799657
GAGCTTA197700.010.53143244
GAGGTCA285250.010.3247285
GTCACTC269900.010.3122648
ACTCTCC281400.09.92269111
TGGCATA209350.09.80295334
CATAGGG223000.09.34435537
CACTCTC303650.09.30686810
TCACTCT319650.09.1225769
GCATAGG227350.09.0367336
CGTGGCG119200.08.7949661
TCAGACG87150.08.4932
ATATAGC46850.08.3565296
TGGGAGG266150.08.3092082
Produced by FastQC (version 0.11.5)