FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45748288
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG27550.022.8625666
CGGTATA39050.020.34200120
TCGACGA35550.017.9076357
CGTTACG25650.017.72367921
AGACCGC106150.017.3568154
CGACGAG35600.017.0610288
CAGACCG113200.016.514323
AACGCTA102350.016.07754123
GAACGAT116400.015.95826145
ACGCTAC104700.015.86719824
CCGTACG67800.015.6609614
CGTACGC69950.015.27595
CGCTACC110600.014.8579325
GTTCGAC41100.014.7782455
ACGTTCG10150.014.4057516
CTCCGAT43400.013.8964721
CCCGTAT27700.013.8111111
AAAACGC120250.013.77471521
TACGCCA79250.013.5681317
GAAAACG124500.013.46563820
Produced by FastQC (version 0.11.5)