FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43069294
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG127100.013.63651245
ATAGGGG126550.013.39164638
GGCATAG129500.012.979897535
GGGAGGT201750.012.1883773
GAGCTTA142000.011.95200144
GTTGTCG20050.011.55647226
GGAGGTC212100.011.28692154
CATAGGG151250.011.23353437
TGGCATA154150.010.99122734
GGTCACT209250.010.9896197
GCATAGG159850.010.69901136
GTCACTC225200.010.2312538
TTACGCG9950.010.17676835
TCAGACG70650.09.9669642
TCACTCT260250.09.259689
ATATAGC36100.09.1000316
TAATCGG33550.08.9193594
GAGGTCA281800.08.8787215
CTAGAAT59100.08.7933161
TGGGAGG213850.08.7843142
Produced by FastQC (version 0.11.5)