FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54709933
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1963930.3589713772817086No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA628020.11479085525474872No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG626150.11444905260622416No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT303000.030.83183345
AGAACGA388200.024.06540544
AGACCGC440650.021.1022154
ACCGCGT451700.020.5811216
CAGACCG454000.020.5758763
CCGCGTT455700.020.3949347
CGCGTTC455950.020.3837498
GACCGCG460350.020.160065
AAGAACG466700.020.02381743
TACGGAG486900.019.23789834
TCAGACC492350.019.215432
ATACGGA487650.019.20841633
AATACGG491800.019.04248232
CAATACG502050.018.66243231
GCGTTCT502750.018.5578849
TTCGACG24050.018.5228986
ACGGAGA515950.018.18537135
TTCGGCG81650.017.63661244
CGGAGAG531450.017.56264536
CCCAATA543550.017.2913729
Produced by FastQC (version 0.11.5)