FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54709933
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG27450.012.70569226
AGCTTAG117250.011.38074545
ATAGGGG121700.011.207108538
GGCATAG121100.011.075850535
GTCAGGA214050.010.6749751
CATAGGG134750.010.05496337
GGGAGGT233050.09.6316623
GCATAGG140150.09.60256936
CGTAGGG67200.09.411303537
GCGTAGG67950.09.3729536
GGCGTAG70550.09.18693135
GAGCTTA150150.09.127644
TGGCATA150150.09.11279534
TGTCGTT38350.09.09479228
ATAACGC67550.08.62952239
GGAGGTC251900.08.5271394
TCAGACG84450.08.521612
GCTAAGT44800.08.486958514
AGCATAG81450.08.39868545
CGTGGCG120350.08.18495751
Produced by FastQC (version 0.11.5)