FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200898

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200898
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47118573
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT725280.1539265630985896No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT161350.021.44760945
AGACCGC169400.020.5854554
TTCGACG14900.019.4780566
CAGACCG183850.019.0409283
CGGATAC106250.017.8501073
CCCGGAT153250.017.8147221
GCCGGCT139550.017.6443731
CCGGCTT170800.017.4661772
AGAACGA200850.017.20687944
CCGGATA117300.016.9357662
TCACGCT159150.016.9070078
TTCGCCA109800.016.80184214
TTCACGC162900.016.7387777
AACGCTA156000.016.34265523
ACGCTAC156250.016.31673224
TACGGAG212600.016.21432934
ATACGGA215250.016.02492533
AAGAACG219400.015.74096843
GCGTTCT222900.015.72538959
AATACGG221700.015.670979532
Produced by FastQC (version 0.11.5)