FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47118573
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG493160.1046636110987487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG159350.015.23529745
GTTGTCG19850.014.50889226
GGGAGGT240700.014.1431383
ATAGGGG174300.013.99312938
GGCATAG176450.013.6441235
GGTCACT250500.013.0329477
GAGCTTA188700.013.00870144
GTCACTC261450.012.7366738
ACTCTCC264850.012.55616311
CATAGGG207550.011.71888737
GGAGGTC296650.011.3846514
AGGTCAC302050.011.2183646
GCATAGG219250.011.09352336
TCACTCT310700.010.9784469
GAGGTCA312150.010.9490855
CACTCTC301550.010.93849510
TGGGAGG239200.010.88316252
TGGCATA224900.010.81482934
ATATAGC33650.010.4977926
TAGGCTA65850.010.4555910
Produced by FastQC (version 0.11.5)