FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47015023
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA820050.17442297114264946No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG723220.15382742660787382No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA687340.1461958234073394No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG662140.14083583453739884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCTA194750.017.4698645
CATGCAT108800.016.683769
TAGCGCG850.002576736715.87353415
GGAGGTC289750.015.5739054
GGGAGGT301000.015.0006293
GTTGTCG47750.014.93329326
CGCGCGA6400.014.42786843
CGTAACG16900.013.98709142
ATATAGC57750.013.7982546
TACATGC138850.013.7616547
ATACATG144500.013.3968736
CGATCCG7000.013.1840941
TAATCGG134000.013.1175524
GAGGTCA349350.013.0722015
TGGGAGG306150.013.042482
AATCGGT141350.012.9615215
CGCGAAA8100.012.79566245
ATAATCG144500.012.7729443
GCTAAGT65200.012.69244414
TGCATTC146950.012.65467111
Produced by FastQC (version 0.11.5)